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An evaluation of BMP‐2 delivery from scaffolds with miniaturized dental implants in a novel rat mandible model

Identifieur interne : 004981 ( Main/Exploration ); précédent : 004980; suivant : 004982

An evaluation of BMP‐2 delivery from scaffolds with miniaturized dental implants in a novel rat mandible model

Auteurs : Bo Wen [République populaire de Chine] ; Matthias Karl [Allemagne] ; David Pendrys [États-Unis] ; David Shafer [États-Unis] ; Martin Freilich [États-Unis] ; Liisa Kuhn [États-Unis]

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RBID : ISTEX:DF96F0672C25E0D14378D8B85D1DF4CF54B87E24

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English descriptors

Abstract

The purpose of this study was three‐fold: (a) to develop a new small animal model to evaluate dental implant systems that recapitulates aspects of the challenging intraoral environment, (b) screen several scaffolds for in vivo bone forming efficacy when used to deliver non‐glycosylated bone morphogenetic protein‐2 (BMP‐2) together with a miniaturized titanium (Ti) dental implant, and (c) identify correlations between in vitro BMP‐2 release rates and in vivo results. The scaffolds tested were: (1) collagen‐hydroxyapatite composite (Col/HA), (2) polyethylene glycol hydrogel (PEG‐hydrogel), and (3) Col/HA infused with PEG‐hydrogel (Col/HA/PEG‐hydrogel). BMP‐2 delivery directly from the Ti implants rather than from the scaffolds was also tested. MicroCT analyses at 4 weeks showed that the maximum volume and height of new bone occurred when BMP‐2 (10 μg) was delivered from the Col/HA/PEG‐hydrogel scaffolds. BMP‐2 delivery from the Ti implant was not as effective as from the scaffolds. While in vitro BMP‐2 release was highest for the PEG‐hydrogel, the scaffold most successful in vivo was the Col/HA/PEG‐hydrogel scaffold because it had the necessary mechanical strength to perform well in the mandibular bone environment. The in vitro release studies suggested a threshold dose of 5 μg which was borne out by the in vivo dose response studies. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.

Url:
DOI: 10.1002/jbm.b.31817


Affiliations:


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<term>Vertical bone growth</term>
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<term>Biomaterial scaffolds</term>
<term>Biomaterials</term>
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<term>Biomedical materials research</term>
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<term>Bone density</term>
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<term>Bone growth</term>
<term>Bone height</term>
<term>Bone marrow aspirate</term>
<term>Bone regeneration</term>
<term>Bone volume</term>
<term>Circumferential manner</term>
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<term>Connecticut health center</term>
<term>Delivery systems</term>
<term>Dental implant</term>
<term>Dental implants</term>
<term>Dental medicine</term>
<term>Different scaffolds</term>
<term>Different test groups</term>
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<term>Dose response study</term>
<term>Drug deliv</term>
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<term>Growth factor</term>
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<term>Hydrogel</term>
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<term>Implant combination</term>
<term>Implant placement</term>
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<div type="abstract" xml:lang="en">The purpose of this study was three‐fold: (a) to develop a new small animal model to evaluate dental implant systems that recapitulates aspects of the challenging intraoral environment, (b) screen several scaffolds for in vivo bone forming efficacy when used to deliver non‐glycosylated bone morphogenetic protein‐2 (BMP‐2) together with a miniaturized titanium (Ti) dental implant, and (c) identify correlations between in vitro BMP‐2 release rates and in vivo results. The scaffolds tested were: (1) collagen‐hydroxyapatite composite (Col/HA), (2) polyethylene glycol hydrogel (PEG‐hydrogel), and (3) Col/HA infused with PEG‐hydrogel (Col/HA/PEG‐hydrogel). BMP‐2 delivery directly from the Ti implants rather than from the scaffolds was also tested. MicroCT analyses at 4 weeks showed that the maximum volume and height of new bone occurred when BMP‐2 (10 μg) was delivered from the Col/HA/PEG‐hydrogel scaffolds. BMP‐2 delivery from the Ti implant was not as effective as from the scaffolds. While in vitro BMP‐2 release was highest for the PEG‐hydrogel, the scaffold most successful in vivo was the Col/HA/PEG‐hydrogel scaffold because it had the necessary mechanical strength to perform well in the mandibular bone environment. The in vitro release studies suggested a threshold dose of 5 μg which was borne out by the in vivo dose response studies. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.</div>
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